Isolation, characterization, and decolorization of Disperse Blue 60 by newly isolated bacterial strains from Kashan...

Wastewaters are a source of water pollution and the environment. Therefore, the decolorization ability of isolated strains from textile wastewater for Disperse Blue 60 dye was measured. After sampling from different parts of textile waste, four decolorizer strains were isolated. The effects of various factors were evaluated on decolorization. Decolorization ability of strains was measured after incubation up to 72 hr, the range of pH 6-9, different dye concentrations from 50 to 400 mg/L, and different carbon sources. The four newly bacterial strains showed high decolorization ability to dye. The highest decolorization (90%-95%) was observed after 72 hr, pH 7-9, 50 mg/L concentration of dye and glucose as carbon source. According to findings from the present study, the decolorization ability of F52, C43, C19, and C25 strains was 93%, 96%, 100%, and 98.33%, respectively. The phytotoxicity test confirmed the nontoxic effect of the dye decolorization products compared with the toxic Disperse Blue 60 on seeds of Raphanus sativus L. Based on the biochemical characterization and 16s rDNA gene sequencing analysis, the selected bacterial strains were identified as Alishewanella, Halomonas, Jonesia, and Pseudomonas genera. High decolorization ability of the bacterial isolates showed they could effectively use in the biological treatment of wastewater. PRACTITIONER POINTS: Isolation of powerful and effective strains from Kashan textile wastewater Increasing of decolorization by improving environmental conditions Alishewanella, Halomonas, Jonesia, and Pseudomonas genera can be used for biological treatment of wastewater.