Effects of Bioactive Agents on Dentin Mineralization Kinetics After Dentin Bleaching

Area of Science:

• Biomaterials Science
• Dental Materials Science
• Surface Chemistry

Background:

• Dentin bleaching can compromise its chemical composition and structural integrity.
• Acid conditioning used in dental restorations can further degrade dentin.
• Effective remineralization strategies are needed to repair bleached and conditioned dentin.

Purpose of the Study:

• To evaluate the remineralization effects of Bioglass 45S5 (BG) and Biosilicate (BS) on control dentin (CD) and bleached dentin (BD).
• To assess the impact of BG and BS remineralization on the chemical composition and micro-tensile bond strength (μTBS) of dentin.
• To investigate the chemical interactions at the dentin-adhesive interface after remineralization.

Main Methods:

• Dentin bleaching performed using the walking bleaching technique.
• Remineralization treatment applied using BG or BS solutions.
• Micro-Raman spectroscopy (MRS) used for quantitative analysis of mineral matrix ratios and interface composition.
• Micro-tensile bond strength (μTBS) testing of restored dentin specimens.

Main Results:

• Both BG and BS significantly increased mineral matrix ratios in CD and BD.
• μTBS values were significantly higher for BG-treated CD and for both BG and BS treated BD compared to untreated controls.
• MRS analysis confirmed increased dentin mineral concentration and new interface peaks, indicating chemical interaction.

Conclusions:

• Remineralization of bleached dentin (BD) with BG and BS is an effective therapy.
• This approach restores mineral content lost during bleaching and acid conditioning.
• Remineralization enhances the chemical interaction between dentin and adhesive systems, improving bond strength.