Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Allosteric behavior of artificial compounds.

I Tabushi1, S Kugimiya

  • 1Department of Synthetic Chemistry, Faculty of Engineering, Kyoto University, Japan.

Biomaterials, Artificial Cells, and Artificial Organs
|January 1, 1988
PubMed
Summary

Artificial allosteric molecules, created by linking metal porphyrins, exhibit cooperative binding. This cooperativity in small molecule binding, like CO or O2, is driven by chemical strain release upon initial molecule attachment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation of a novel aggregate like sugar-ball micelle composed of poly(methylglutamate) and poly(ethyleneglycol) modified by lactose and its molecular recognition by lectin.

Chemical & pharmaceutical bulletin·2001
Same author

Poly-L-glutamine forms cation channels: relevance to the pathogenesis of the polyglutamine diseases.

Biophysical journal·2000
Same author

Relationships between structure and molting hormonal activity of tebufenozide, methoxyfenozide, and their analogs in cultured integument system of Chilo suppressalis Walker.

Steroids·2000
Same author

[Artificial enzymes and super-enzymes].

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·1987
Same author

Circular dichroism study of bacteriorhodopsin-lipid interaction.

Biochemical and biophysical research communications·1987
Same author

Attempted chemical construction of artificial single cells.

Annals of the New York Academy of Sciences·1986

Area of Science:

  • Supramolecular Chemistry
  • Coordination Chemistry
  • Chemical Biology

Background:

  • Artificial allosteric molecules are synthesized by covalently linking two independent metal porphyrin units.
  • These dimeric structures mimic monomeric metal porphyrins in most aspects, excluding adsorption-dissociation behaviors.

Purpose of the Study:

  • To investigate the cooperative binding and dissociation of small molecules (e.g., CO, O2, bases) to the metal centers of dimeric porphyrins.
  • To elucidate the role of chemical strain in facilitating cooperative binding events.

Main Methods:

  • Covalent synthesis of dimeric metal porphyrin molecules.
  • Characterization of adsorption and dissociation properties.
  • Analysis of cooperative binding phenomena.

Main Results:

  • Dimeric porphyrins display unique adsorption-dissociation characteristics compared to monomers.
  • Strong cooperativity is observed in the binding of small molecules to the metal centers.
  • Initial small molecule binding induces a coordinating change, releasing pre-existing chemical strain within the dimeric structure.

Conclusions:

  • The study highlights the potential of engineered dimeric porphyrins as artificial allosteric systems.
  • Cooperative binding is a key feature, modulated by the release of chemical strain.
  • These findings offer insights into designing molecules with tunable binding affinities for small molecules.

Related Experiment Videos