Crosslinked on novel nanofibers with thermophilic carbonic anhydrase for carbon dioxide sequestration

The recombinant Sulfurihydrogenibium yellowstonense carbonic anhydrase (SyCA) was covalently bonded on novel polyacrylonitrile (PAN) and polyethylene terephthalate (PET) nanofibers (PAN-PET-PAN donated as AEA) that was first fabricated by electrospinning. The resulting composite materials further crosslinked by the glutaraldehyde, which significantly increased thermostability up to 89.8% and 18.0% after heating at 60 °C for 1 h for immobilized crude and pure SyCA, respectively. After four repetitive attempts in the demonstration of CO₂ sequestration, immobilized crude and pure SyCA on AEA also effectively improved the total CaCO₃ yields to be 5.8 folds and 2.2 folds compared to free enzyme. Furthermore, the endurance of immobilized crude was investigated on flue gasses, which was retained its activity up to 57% on 50 mM NOx and 61% on 50 mM SOx presence. This is the first report of immobilized thermophilic SyCA on a novel nanofiber at the reusability, durability, sequestration of carbon dioxide, tolerant to sulfur oxides (SOx) and nitrogen oxides (NOx) toxic gases and to prevent air pollution.