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Related Experiment Videos

Regenerative responses to exchange perfusion.

L E McCoy1, L A Elliott, C E Lucas

  • 1Department of Physiology, Wayne State University, School of Medicine, Detroit, Michigan 48201.

Biomaterials, Artificial Cells, and Artificial Organs
|January 1, 1988
PubMed
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Perfluorocarbon-based oxygen carriers like Fluosol-DA 20% can cause temporary drops in key blood proteins and platelets. These substances eventually regenerate, but potential risks of thrombosis and immunosuppression warrant further investigation.

Area of Science:

  • Biomedical Engineering
  • Hematology
  • Toxicology

Background:

  • Perfluorocarbon-based oxygen transport fluids (e.g., Fluosol-DA 20%) are investigated for their potential in blood substitution therapies.
  • Understanding the physiological impact of these artificial oxygen carriers on coagulation and immune factors is crucial for safety assessment.

Purpose of the Study:

  • To evaluate the effects of isovolemic exchange perfusion with Fluosol-DA 20% on plasma proteins and blood cell counts in rats.
  • To assess the impact of Fluosol-DA 20% resuscitation on coagulation factors and blood cell recovery following hemorrhagic shock in dogs.

Main Methods:

  • Conscious normal and splenectomized rats underwent isovolemic exchange perfusion to achieve a hematocrit of +/- 3% using Fluosol-DA 20%.
  • Splenectomized dogs were subjected to hemorrhagic shock and resuscitated with Fluosol-DA 20%.

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Main Results:

  • Fluosol-DA 20% caused a significant depression of fibrinogen and plasma globulins in rats, with rapid regeneration observed.
  • In rats, red blood cells and platelets took 14-21 days to normalize, while leukocytes recovered within 1-2 days.
  • Hemorrhagic shock resuscitation in dogs showed selective depression of platelets, plasma globulins, IgG, and fibrinogen, with platelets remaining depressed for 24 hours.

Conclusions:

  • Perfluorocarbon-based oxygen carriers can transiently alter plasma protein levels and blood cell counts.
  • The observed alterations suggest a potential risk of thrombosis and immunosuppression associated with Fluosol-DA 20% administration.