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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
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Characterization and Functional Divergence of a Novel DUF668 Gene Family in Rice Based on Comprehensive Expression

Hua Zhong1, Hongyu Zhang2, Rong Guo2

  • 1State Key Laboratory for Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.

Genes
|December 5, 2019
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Summary

A novel plant gene family, DUF668, was identified in rice and other plants. These genes are crucial for drought tolerance and plant defense mechanisms, responding to various environmental stresses.

Keywords:
DUF668 gene familyexpression patternsphylogenetic analysisricestress

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Area of Science:

  • Plant molecular biology
  • Genomics
  • Plant physiology

Background:

  • The Domain of Unknown Function (DUF) superfamily includes proteins with uncharacterized roles in plants.
  • The DUF668 family, characterized by a 29 amino-acid conserved domain, has not been previously described in plants.

Purpose of the Study:

  • To identify and characterize the novel DUF668 gene family in plants, focusing on rice (Oryza sativa).
  • To investigate the evolutionary conservation, phylogenetic relationships, and functional roles of DUF668 genes in response to environmental stimuli.

Main Methods:

  • Bioinformatic analysis for gene identification, phylogenetic tree construction, and cis-element prediction.
  • Expression profiling of OsDUF668 genes across various rice tissues, hormone treatments, and stress conditions (drought, salt, wound, pathogen).

Main Results:

  • Identified 12 OsDUF668 genes in rice and 91 DUF668 genes across seven other plant species, indicating ancient conservation predating dicot-monocot divergence.
  • Phylogenetic analysis revealed two distinct clades (I and II), with limited gene duplication observed in rice.
  • Over 80% of DUF668 genes possess cis-elements related to phytohormone and light responsiveness.
  • OsDUF668 genes are ubiquitously expressed in rice, with seven showing tissue-specific expression. All OsDUF668 genes responded to drought, and some exhibited rapid altered expression under salt, wound, and rice blast stresses.

Conclusions:

  • The DUF668 gene family is conserved across plant species and plays a significant role in rice development and fitness.
  • OsDUF668 genes are essential for drought tolerance and contribute to plant defense mechanisms against various biotic and abiotic stresses.