Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.1K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.1K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

11.6K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
11.6K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.1K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.1K
Notch Signaling Pathway03:14

Notch Signaling Pathway

6.3K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.3K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

6.1K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.1K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

9.7K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Setmelanotide for the Treatment of Acquired Hypothalamic Obesity.

The New England journal of medicine·2026
Same author

Retinal blood flow increases with diabetic retinopathy severity in type 2 diabetes: a cross-sectional study using laser doppler velocimetry.

Scientific reports·2026
Same author

Deletion of Cab39 adaptors results in KS-WNK1 independent accumulation of SPAK in biomolecular condensates.

American journal of physiology. Renal physiology·2026
Same author

Association Between Hypnotics and Glycemic Variability Assessed by Continuous Glucose Monitoring Under Real-Life Conditions in Patients With Type 2 Diabetes: A Cross-Sectional Study.

Journal of diabetes research·2026
Same author

Lipid-induced degradation of UHMWPE: Clinical assessment of retrieved orthopedic implants, analysis of synovial fluids, and in vitro validation.

Journal of the mechanical behavior of biomedical materials·2026
Same author

Impact of gastric acid suppressants on oral protein kinase inhibitor efficacy: A systematic review and meta-analysis of plasma concentrations and survival outcomes.

Journal of oncology pharmacy practice : official publication of the International Society of Oncology Pharmacy Practitioners·2026

Related Experiment Video

Updated: Jan 2, 2026

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
10:03

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

Published on: August 10, 2014

12.4K

Pin1 Plays Essential Roles in NASH Development by Modulating Multiple Target Proteins.

Masa-Ki Inoue1, Yusuke Nakatsu1, Takeshi Yamamotoya1

  • 1Department of Medical Science, Graduate School of Medicine, Hiroshima University, 1-2-3 Kasumi, Minamitnf-ku, Hiroshima City, Hiroshima 734-8551, Japan.

Cells
|December 5, 2019
PubMed
Summary

Pin1 protein exacerbates non-alcoholic steatohepatitis (NASH) by promoting liver lipid accumulation and fibrosis. Inhibiting Pin1 offers a promising therapeutic strategy for NASH treatment and prevention.

Keywords:
NAFLDNASHPin1fibrosisinflammationlipid

More Related Videos

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
09:02

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Published on: December 10, 2015

7.7K
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

8.9K

Related Experiment Videos

Last Updated: Jan 2, 2026

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
10:03

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

Published on: August 10, 2014

12.4K
Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
09:02

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Published on: December 10, 2015

7.7K
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

8.9K

Area of Science:

  • Biochemistry
  • Hepatology
  • Molecular Biology

Background:

  • Pin1, a prolyl-isomerase, is upregulated in obesity.
  • Pin1 exacerbates hepatic lipid accumulation, inflammation, and fibrosis.

Purpose of the Study:

  • To review the molecular mechanisms of Pin1 in non-alcoholic steatohepatitis (NASH) development.
  • To explore Pin1 inhibition as a therapeutic strategy for NASH and hepatic fibrosis.

Main Methods:

  • Review of existing literature on Pin1 function in liver disease.
  • Analysis of molecular pathways targeted by Pin1 in NASH pathogenesis.

Main Results:

  • Pin1 targets proteins like IRS-1, AMPK, and ACC1 in lipid accumulation.
  • Pin1 influences the NF-kB and TGF-β pathways, contributing to fibrosis.
  • Pin1-deficient mice show resistance to diet-induced NASH.

Conclusions:

  • Increased Pin1 drives both lipid accumulation and fibrotic changes in NASH.
  • Pin1 inhibition is a potential therapeutic avenue for NASH and liver fibrosis.
  • Pin1 deficiency does not impair normal liver function.