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Updated: Jan 2, 2026
A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
Published on: October 11, 2018
[Development of animal experimental model for bacterial peritonitis]
O Montenegro, S Illescas, J C González
1Fco Javier Redondo Calvo, Servicio de Anestesilogía y Reanimación. Unidad de Investigación Traslacional. Hospital General Universitario de Ciudad Real. C/Obispo General Torija s/n.13005. Ciudad Real. Spain. fjredondo@sescam.jccm.es.
Objective:
The aim of the study was to develop a model of abdominal sepsis in the experimental animal.
Methods:
Sprague-Dawley male rats of 5 weeks (N=39) were used. Initially, a pilot study (N = 9) was performed and distributed in 3 groups with 1cc inoculum of Escherichia coli ATCC 25922 intraperitoneally at concentrations of 10E8, 10E9 and 10E10 CFU. Subsequently, concentrations of 10E10 CFU are used in two groups of 3 rats with dilutions of 10 cc and 15 cc of distilled water respectively. Finally, a randomized trial of 24 rats was started in three treatment groups after intraperitoneal infection: Group I with physiological serum (N = 6), Group II with ceftriaxone (N = 9), Group III with ceftriaxone plus allicin (N = 9). Microbiological samples of blood and peritoneal fluid were made, as well as histopathological study of intraperitoneal organs (liver, diaphragm and peritoneum).
Results:
Death of 100% of the rats infected with 10E10 E. coli UFC concentration with the dilution of 15 ml of distilled water and without antibiotic was oberved. The blood culture and peritoneal fluid culture was positive for the same strain in all of them. The formation of abscesses on the liver surface and polymorphonuclear infiltration in tissues were observed.
Conclusions:
The lethal dose of E. coli is 10E10 CFU diluted in 15 cc distilled water by intraperitoneal injection.
