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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

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Quantitative Proteomic Profiling of Cryptococcus neoformans.

Brianna Ball¹, Jennifer Geddes-McAlister¹

¹Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.

Current Protocols in Microbiology

|December 5, 2019

Summary

This study details methods for analyzing Cryptococcus neoformans proteins, aiding the search for new antifungal treatments against this opportunistic fungal pathogen.

Keywords:

Cryptococcus neoformans computational proteomics fungal pathogenesis mass spectrometry-based proteomics secretome total proteome

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Identification of protein complexes with quantitative proteomics in S. cerevisiae

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Published on: March 4, 2009

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

Area of Science:

Medical Mycology
Proteomics
Infectious Diseases

Background:

Cryptococcus neoformans is an opportunistic pathogen causing life-threatening meningoencephalitis, particularly in immunocompromised individuals.
Virulence factors include capsule, melanin, and enzymes; traditional analysis methods are supplemented by advanced proteomics.
Antifungal resistance necessitates novel therapeutic strategies.

Purpose of the Study:

To outline comprehensive proteomic profiling protocols for Cryptococcus neoformans.
To investigate protein abundance changes in cellular and extracellular compartments.
To identify potential antivirulence targets and therapeutic options.

Main Methods:

Detailed protocols for culturing and sample preparation of C. neoformans.
Methods for protein extraction from both cell pellets and culture supernatants.
Proteomic profiling using mass spectrometry and bioinformatics analysis.

Main Results:

Established standardized protocols for C. neoformans proteome and secretome analysis.
Enabled comprehensive profiling of fungal cellular and secreted proteins.
Provided a foundation for identifying proteins responsive to environmental conditions.

Conclusions:

Proteomics offers powerful insights into C. neoformans virulence and host-pathogen interactions.
Defined protocols facilitate the discovery of novel antivirulence strategies.
This work supports the development of alternative therapies against cryptococcosis.