Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

System configuration optimization for mesoscopic fluorescence molecular tomography.

Fugang Yang1,2, Denzel Faulkner3, Ruoyang Yao3

  • 1School of Information and Electronic Engineering, Shandong Technology and Business University, Yantai 264005, China.

Biomedical Optics Express
|December 5, 2019
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The association between sedentary behavior and infertility: a population-based cross-sectional observational study and Mendelian randomization analysis.

Reproductive biology and endocrinology : RB&E·2025
Same author

Comparative effects of acupuncture and metformin on insulin sensitivity in women with polycystic ovary syndrome: a systematic review and meta-analysis.

Frontiers in endocrinology·2025
Same author

Design and characterization of an optical phantom for mesoscopic multimodal fluorescence lifetime imaging and optical coherence elastography.

Biomedical optics express·2025
Same author

Fluorescence Lifetime Imaging for Quantification of Targeted Drug Delivery in Varying Tumor Microenvironments.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024
Same author

Effects of the Zishen Yutai Pill on live births compared with placebo among infertile women with frozen-thawed embryo transfer cycle: A multicentre double-blind randomized controlled trial.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2024
Same author

Analysis of weighted gene co-expression networks and clinical validation identify hub genes and immune cell infiltration in the endometrial cells of patients with recurrent implantation failure.

Frontiers in genetics·2024
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Mesoscopic Fluorescence Molecular Tomography (MFMT) enables high-resolution 3D imaging of deep tissue engineering constructs. A 2D detection system in de-scanned mode offers optimal performance for fluorescence biomarker reconstruction.

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Tissue Engineering

Background:

  • Tissue engineering requires non-invasive, 3D imaging of bioprinted constructs for longitudinal assessment.
  • Thick, turbid tissue constructs challenge traditional fluorescence microscopy.
  • Need for advanced imaging techniques to visualize biomarkers within engineered tissues.

Purpose of the Study:

  • To develop and optimize a Mesoscopic Fluorescence Molecular Tomography (MFMT) methodology.
  • To enable high-resolution 3D tomographic reconstruction of fluorescence biomarkers at millimeter depths.
  • To investigate the impact of optical system configurations on MFMT imaging performance.

Main Methods:

  • Developed an MFMT system utilizing an inverse problem approach for tomographic reconstruction.

Related Experiment Videos

  • Investigated critical technical parameters: optode sampling, forward model design, and inverse solver settings.
  • Evaluated the influence of detector layout, detector/source numbers, and scanning modes (coupled/uncoupled arrays).

    • Main Results:

    • MFMT successfully enabled high-resolution reconstruction of fluorescence biomarkers at millimeter depths.
    • Imaging performance was highly dependent on optical system configuration parameters.
    • A 2D detection chain implemented in a de-scanned mode demonstrated optimal imaging reconstruction.

    Conclusions:

    • MFMT is a promising technique for 3D imaging of challenging tissue engineering constructs.
    • Optimization of optical system parameters is crucial for achieving high-quality tomographic reconstructions.
    • The proposed MFMT system with a 2D de-scanned detection array provides superior imaging performance.