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Up-regulated microRNA-33b inhibits epithelial-mesenchymal transition in gallbladder cancer through down-regulating

Guohui Xu1, Xiaoyong Wei1, Qiang Tu1

  • 1Department of Hepatobiliary Tumor Surgery, Jiangxi Cancer Hospital, Nanchang 330029, P. R. China.

Bioscience Reports
|December 5, 2019
PubMed

Insights

MicroRNA-33b (miR-33b) acts as a tumor suppressor in gallbladder cancer (GBC) by down-regulating CROCC. This inhibition impedes epithelial-mesenchymal transition (EMT), hindering GBC progression and offering a potential new treatment target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gastroenterology

Background:

  • Gallbladder cancer (GBC) is a rare but aggressive malignancy.
  • MicroRNA-33b (miR-33b) is a known tumor suppressor in various cancers.
  • CROCC is a potential target of miR-33b, implicated in cancer progression.

Purpose of the Study:

  • To investigate the role of miR-33b in GBC.
  • To elucidate the mechanism of miR-33b in regulating epithelial-mesenchymal transition (EMT) in GBC.
  • To determine if miR-33b targets CROCC in GBC.

Main Methods:

  • GBC cell lines were treated with miR-33b mimics/inhibitors or siRNA targeting CROCC.
  • Expression levels of miR-33b, CROCC, and EMT-related genes (E-cadherin, N-cadherin, Vimentin) were assessed.
  • Cell proliferation, migration, and invasion assays were performed.

Main Results:

  • miR-33b was underexpressed, while CROCC was overexpressed in GBC cells, correlating with enhanced EMT.
  • miR-33b was confirmed to target and down-regulate CROCC expression.
  • Upregulating miR-33b or silencing CROCC increased E-cadherin and decreased N-cadherin and Vimentin, inhibiting proliferation, migration, invasion, EMT, and tumor growth.

Conclusions:

  • miR-33b suppresses GBC development by down-regulating CROCC and inhibiting EMT.
  • Targeting the miR-33b/CROCC axis presents a promising therapeutic strategy for GBC.
Keywords:
CROCCEpithelial-mesenchymal transitionGallbladder cancerInvasionProliferationmicroRNA-33b

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