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Long noncoding RNA SNHG14 accelerates cell proliferation, migration, invasion and suppresses apoptosis in colorectal

Q Pei1, G-S Liu, H-P Li

  • 1Department of Gastroenterology Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China. ni1967829e@126.com.

European Review for Medical and Pharmacological Sciences
|December 5, 2019
PubMed
Abstract

Insights

The long non-coding RNA SNHG14 promotes colorectal cancer (CRC) progression by targeting miR-944 and KRAS, impacting the PI3K/AKT pathway. This finding offers potential new biomarkers for CRC therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gastroenterology

Background:

  • Colorectal cancer (CRC) is a significant global health concern, characterized by high metastasis rates.
  • The role of nuclear RNA host gene 14 (SNHG14) as an oncogene is recognized, but its specific regulatory mechanism in CRC remains largely unknown.

Purpose of the Study:

  • To elucidate the regulatory mechanism of SNHG14 in colorectal cancer development.
  • To investigate the interaction between SNHG14, miR-944, and KRAS in CRC.

Main Methods:

  • Quantitative Real-Time PCR (qRT-PCR) and Western blotting were used to assess gene and protein expression.
  • Cell viability, apoptosis, migration, and invasion assays (CCK-8, flow cytometry, Transwell) were performed.
  • In vivo tumor growth was evaluated in animal models, and molecular interactions were confirmed via luciferase reporter and RIP assays.

Main Results:

  • SNHG14 and KRAS were upregulated, while miR-944 was downregulated in CRC tissues and cells.
  • SNHG14 silencing inhibited CRC cell proliferation, migration, and invasion, and promoted apoptosis by suppressing the PI3K/AKT pathway.
  • MiR-944 directly targeted KRAS, and its inhibition reversed the effects of SNHG14 silencing on CRC cells.

Conclusions:

  • SNHG14 promotes CRC cell proliferation, migration, and invasion while suppressing apoptosis.
  • The SNHG14/miR-944/KRAS axis regulates CRC progression via the PI3K/AKT pathway.
  • SNHG14 represents a potential novel biomarker for CRC diagnosis and therapy.

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