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Long non-coding RNA TOB1-AS1 modulates cell proliferation, apoptosis, migration and invasion through miR-23a/NEU1

K Jiang1, X-H Zhi, Y-Y Ma

  • 1Department of Radiation Oncology, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an No. 2 Hospital, Jiangsu, China. zlq_hill@126.com.

European Review for Medical and Pharmacological Sciences
|December 5, 2019
PubMed
Abstract

Insights

Long non-coding RNA TOB1 antisense RNA 1 (TOB1-AS1) is downregulated in gastric cancer (GC). Upregulating TOB1-AS1 or NEU1 inhibits GC progression by targeting miR-23a via the Wnt/β-catenin pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GC) is a significant global health challenge.
  • The role of long non-coding RNA TOB1 antisense RNA 1 (TOB1-AS1) in GC progression remains unclear.

Purpose of the Study:

  • To elucidate the precise role of TOB1-AS1 in gastric cancer.
  • To investigate the molecular mechanisms underlying TOB1-AS1's function in GC.

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blot to assess gene and protein expression.
  • Cellular assays including MTT, flow cytometry, and Transwell assays to evaluate proliferation, apoptosis, migration, and invasion.
  • Dual-Luciferase reporter assay to determine interactions between TOB1-AS1, miR-23a, and NEU1, with in vivo validation.

Main Results:

  • TOB1-AS1 levels were significantly decreased in GC tissues and cell lines.
  • Upregulation of TOB1-AS1 or lysosomal sialidase (NEU1) inhibited GC cell proliferation, migration, and invasion while promoting apoptosis.
  • TOB1-AS1 acts as a sponge for miR-23a, which in turn targets NEU1, collectively regulating the Wnt/β-catenin signaling pathway.

Conclusions:

  • The TOB1-AS1/miR-23a/NEU1 axis plays a crucial role in regulating GC cell proliferation, apoptosis, migration, and invasion via the Wnt/β-catenin pathway.
  • TOB1-AS1 demonstrates potential as a therapeutic target for human gastric cancer.

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