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Electrochemical MIP Sensor for Butyrylcholinesterase
Goksu Ozcelikay1,2, Sevinc Kurbanoglu1, Xiaorong Zhang2
1Faculty of Pharmacy, Department of Analytical Chemistry, Ankara University, Tandogan, Ankara 06560, Turkey.
Polymers
|December 6, 2019
Summary
Researchers developed a novel molecularly imprinted polymer (MIP) sensor for detecting butyrylcholinesterase (BuChE), a key diagnostic enzyme. This sensitive sensor offers a new tool for BuChE analysis in various applications.
Area of Science:
- Biomimetic sensors
- Analytical chemistry
- Polymer science
Background:
- Molecularly imprinted polymers (MIPs) offer antibody-like recognition capabilities by utilizing synthetic polymers.
- Butyrylcholinesterase (BuChE) is a diagnostically significant enzyme requiring sensitive detection methods.
Purpose of the Study:
- To create the first MIP specifically designed for the recognition of butyrylcholinesterase (BuChE).
- To develop a sensitive electrochemical sensor for BuChE detection and activity measurement.
Main Methods:
- Electropolymerization of o-phenylenediamine to create a MIP thin film on a glassy carbon electrode.
- Detection of BuChE rebinding and removal using cyclic voltammetry with ferricyanide redox marker.
- Measurement of enzymatic activity via anodic oxidation of thiocholine.
Main Results:
- A linear response for BuChE detection was observed between 50 pM and 2 nM.
- A highly sensitive detection limit of 14.7 pM for BuChE was achieved.
- The sensor demonstrated responsiveness to pseudo-irreversible BuChE inhibitors in the millimolar range.
Conclusions:
- The developed MIP sensor provides a highly sensitive and selective method for BuChE detection.
- This sensor platform holds potential for diagnostic applications and inhibitor screening.
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