Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Attitude and practices of district-level HMIS managers towards malaria routine reporting in Uganda: a 2024 cross-sectional study.

Malaria journal·2026
Same author

Modeling and Predicting Counts and Environmental Correlates of Mosquito Distributions in Haiti Using Surveillance Data from the Ouest Department.

The American journal of tropical medicine and hygiene·2026
Same author

Continental-scale genomic surveillance of Plasmodium falciparum malaria across sub-Saharan Africa with rapid nanopore sequencing.

Nature communications·2026
Same author

Assessing the effect of malaria exposure history on tetanus antibody waning rates among children in Jinja and Tororo Districts, Uganda.

The Journal of infectious diseases·2026
Same author

Malaria antibody responses augment surveillance in low-transmission settings in the Upper River Region, the Gambia.

Frontiers in epidemiology·2026
Same author

Parasite and gametocyte dynamics and infectivity to mosquitoes during recurrent Plasmodium vivax infections: A longitudinal study from Ethiopia.

The Journal of infection·2026

Related Experiment Video

Updated: Jan 2, 2026

A Multi-detection Assay for Malaria Transmitting Mosquitoes
09:00

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

13.6K

High-throughput malaria serosurveillance using a one-step multiplex bead assay.

Eric Rogier1, Lotus van den Hoogen2, Camelia Herman3,4

  • 1Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, 30329, USA. erogier@cdc.gov.

Malaria Journal
|December 6, 2019
PubMed
Summary

A novel overnight incubation method for malaria antibody detection significantly reduces lab time and enhances signal detection. This efficient approach aids large-scale serosurveys for accurate malaria exposure data.

Keywords:
MalariaMultiplex immunoassayProtocolSeroprevalence

More Related Videos

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.8K
Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay
07:04

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

Published on: September 30, 2021

2.6K

Related Experiment Videos

Last Updated: Jan 2, 2026

A Multi-detection Assay for Malaria Transmitting Mosquitoes
09:00

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

13.6K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.8K
Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay
07:04

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

Published on: September 30, 2021

2.6K

Area of Science:

  • Immunology
  • Infectious Diseases
  • Biotechnology

Background:

  • Serological data reveals population exposure and transmission patterns of infectious diseases.
  • Traditional immunoassays for large-scale serosurveys are time-consuming due to multi-step protocols.
  • Malaria serosurveillance is increasingly important for accurate population exposure estimates.

Purpose of the Study:

  • To develop and evaluate a novel, time-efficient bead-based immunoassay for antibody detection.
  • To assess the impact of a concurrent overnight incubation protocol on assay performance for malaria antigens.

Main Methods:

  • A Luminex multiplex bead-based assay (MBA) was utilized to detect IgG antibodies against eight malaria antigens.
  • Sera and dried blood spots from a Haitian serosurvey were tested using both standard multi-step and a novel OneStep overnight incubation protocol.
  • The OneStep assay involved concurrent incubation of samples and all reagents.

Main Results:

  • The OneStep assay significantly amplified IgG detection signals for all eight malaria antigens compared to the standard assay.
  • Enhanced signal amplification was most pronounced at low- and mid-range IgG titers, indicating improved analyte detection.
  • Seroprevalence estimates remained consistent across both assay protocols and sample types (serum vs. dried blood spots).

Conclusions:

  • Concurrent overnight incubation in MBA assays minimizes laboratory hands-on time for staff.
  • The OneStep assay enhances IgG signal detection for multiple malaria antigens.
  • This optimized protocol is suitable for high-throughput, large-scale malaria serosurveys, enabling timely antibody data collection.