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The Long Non-Coding RNA SBF2-AS1 Exerts Oncogenic Functions In Gastric Cancer By Targeting The miR-302b-3p/E2F

Chaojie Liang1, Chaosen Yue2, Chaowei Liang1

  • 1Department of General Surgery, First Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, Shanxi 030001, People's Republic of China.

Oncotargets and Therapy
|December 6, 2019
PubMed
Abstract

Insights

The long non-coding RNA SBF2-AS1 is overexpressed in gastric cancer (GC), promoting tumor progression. Targeting SBF2-AS1 may offer a new diagnostic and prognostic strategy for GC patients.

Area of Science:

  • Molecular Oncology
  • Cancer Genomics

Background:

  • Long non-coding RNA SBF2-AS1 is implicated in cancer progression.
  • Its specific role in gastric cancer (GC) remains largely unexplored.

Purpose of the Study:

  • To investigate the mechanism of SBF2-AS1 in gastric cancer.
  • To evaluate SBF2-AS1 as a diagnostic and prognostic biomarker for GC.

Main Methods:

  • Meta-analysis of public datasets (GEO, TCGA) for prognostic value.
  • RT-PCR for clinicopathologic correlation.
  • In vitro gain/loss-of-function assays in GC cell lines.
  • Luciferase reporter and bioinformatics for target validation.

Main Results:

  • SBF2-AS1 is significantly overexpressed in GC tissues and cell lines.
  • High SBF2-AS1 expression correlates with poor overall survival and serves as an independent prognostic factor.
  • SBF2-AS1 knockdown inhibits GC cell growth, invasion, and metastasis while promoting apoptosis and cell cycle arrest.
  • SBF2-AS1 functions as a competing endogenous RNA (ceRNA) for miR-302b-3p, thereby inhibiting miR-302b-3p's suppression of E2F3.

Conclusions:

  • SBF2-AS1 is a potential diagnostic and prognostic biomarker for gastric cancer.
  • SBF2-AS1 promotes GC progression through the miR-302b-3p/E2F3 signaling axis.
Keywords:
E2F3SBF2-AS1gastric cancermiR-302b-3p

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