Polymorphism of MPT64 and PstS1 in Mycobacterium tuberculosis is not likely to affect relative immune reaction in

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Tongyang Xiao1,2,3, Yi Jiang1,2, Guilian Li1,2

  • 1State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing.

Medicine
|December 6, 2019
PubMed
Abstract

Insights

Mycobacterium tuberculosis antigens MPT64 and PstS1 show promise for tuberculosis diagnosis using the T-spot test. Their genetic variations had minimal impact on immune responses, suggesting reliability in diagnostic applications.

Area of Science:

  • Immunology
  • Microbiology
  • Diagnostic Development

Background:

  • MPT64 and PstS1 are key immune-dominant antigens of Mycobacterium tuberculosis.
  • These antigens are frequently explored as candidates for tuberculosis diagnosis.

Purpose of the Study:

  • To evaluate the diagnostic potential of MPT64 and PstS1 antigens from Mycobacterium tuberculosis.
  • To assess the impact of MPT64 and PstS1 polymorphisms on immune responses for tuberculosis diagnostics.

Main Methods:

  • Recombinant plasmids were constructed to express wild and mutant forms of MPT64 and PstS1.
  • Purified proteins were used as antigens in T-spot and ELISA assays.
  • Samples were collected from 96 tuberculosis patients, 53 non-tuberculosis individuals, and 96 healthy volunteers.

Main Results:

  • T-spot assays showed promising Area Under the Curve (AUC) values for PstS1 (0.745–0.817) and MPT64 (0.723–0.750) antigens.
  • ELISA assays yielded lower AUC values for both MPT64 (0.525–0.528) and PstS1 (0.509–0.588).
  • No significant difference in diagnostic performance was observed between wild and mutant MPT64 and PstS1 proteins.

Conclusions:

  • MPT64 and PstS1 are viable candidate antigens for Mycobacterium tuberculosis T-spot diagnostic tests, potentially in combination with other antigens.
  • Polymorphisms in MPT64 and PstS1 exhibit minimal influence on host cell-mediated and humoral immunity.

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