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Inhibition of human cervical cancer cell invasion by IL-37 involving runt related transcription factor 2 suppression

Ping Ouyang1, Kun Wu1,2, Liudan Su1,2

  • 1Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan 523808, China.

Annals of Translational Medicine
|December 7, 2019
PubMed
Abstract

Insights

Interleukin-37 (IL-37) inhibits human cervical cancer cell invasion by suppressing RUNX2 expression. This finding highlights IL-37 as a potential therapeutic target for cervical cancer treatment.

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Interleukin-37 (IL-37) is an anti-inflammatory cytokine with largely unknown roles in cancer.
  • Preliminary studies suggest IL-37 inhibits cervical cancer (CC) cell invasion and affects RUNX family gene expression.
  • The function of RUNX family genes in CC is also not well understood.

Purpose of the Study:

  • To investigate the effects of IL-37 on cell invasion in CC cell lines.
  • To determine the impact of IL-37 on the expression of runt related transcription factor 2 (RUNX2) in CC cells.

Main Methods:

  • Transfection of Siha and C33A cells with plasmids overexpressing IL-37 or RUNX2.
  • Quantitative real-time PCR to detect RUNX1, RUNX2, and RUNX3 mRNA expression.
  • Western blot analysis for protein expression and grayscale scanning.
  • Transwell assays to evaluate cell invasion.

Main Results:

  • IL-37 suppressed RUNX1 and RUNX2 mRNA expression while increasing RUNX3 mRNA levels.
  • Overexpression of IL-37 significantly inhibited cell invasion in both Siha and C33A cell lines.
  • IL-37 suppressed both mRNA and protein expression of RUNX2.
  • Overexpression of RUNX2 partially reversed the inhibitory effect of IL-37 on cell invasion.

Conclusions:

  • IL-37 significantly inhibits the invasion of cervical cancer cells (Siha and C33A).
  • This inhibitory effect of IL-37 on cell invasion is mediated through the suppression of RUNX2 expression.
Keywords:
IL-37cell invasioncervical cancer (CC)cytokinerunt related transcription factor 2 (RUNX2)tumor

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