Phylogenetic analyses and pathogenicity of a variant infectious bursal disease virus strain isolated in China

Infectious bursal disease is an acute, highly contagious, immunosuppressive disease of young chickens caused by infectious bursal disease virus (IBDV). In recent years, there has been a notable increase in the isolation rates of variant IBDV strains in China; however, the pathogenicity of these variants is unclear. In the current study, we characterized variant IBDV strain ZD-2018-1 and assessed its pathogenicity in specific-pathogen-free chickens. Phylogenetic analysis revealed that ZD-2018-1 belonged to the variant IBDV strain, which showed several unique amino acid mutations compared with other previously-isolated variant IBDV strains. Pathogenicity assays showed that ZD-2018-1 was less virulent than very virulent IBDV strain SD-2013-1, and did not cause an obvious symptoms or death. In comparison, strain SD-2013-1 had a high mortality rate and caused severe lesions in various tissues. However, both of the strains induced obvious pathological lesions on the bursa of Fabricius, resulting in severe immunosuppression in the infected chickens. The results of this study present a systematic evaluation of the genetic characteristics, pathogenicity, and immunosuppressive properties of a new variant IBDV strain, and may help in the development of strategies for the prevention and control of IBDV in poultry.