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Updated: Jan 2, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
Simplified monomeric VHH-Fc antibodies provide new opportunities for passive immunization
Henri De Greve1, Vikram Virdi2, Shruti Bakshi3
1Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium; VIB Center for Structural Biology, 1050 Brussels, Belgium.
Abstract:
Simplified monomeric monoclonal antibodies consisting of a single-domain VHH, derived from camelid heavy-chain only antibodies, fused with the Fc domain of either IgG (VHH-IgG) or IgA (VHH-IgA) antibodies, are promising therapeutic proteins. These simplified single-gene encoded antibodies are much easier to manufacture and can be produced in plants and in yeast for bulk applications. These merits enable novel passive immunization applications, such as in-feed oral delivery of VHH-IgAs, which have successfully provided protection against a gastrointestinal infection in the piglet model.
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