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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Jan 2, 2026

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

833

Author Correction: Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base

Beau R Webber1,2,3,4, Cara-Lin Lonetree1,2,3, Mitchell G Kluesner1,2,3

  • 1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA.

Nature Communications
|December 8, 2019
PubMed
Summary
This summary is machine-generated.

This study has a published amendment. Please find the link at the top of the paper for the updated version.

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Area of Science:

  • Not specified in the abstract.

Context:

  • An amendment to this paper has been published.

Purpose:

  • To provide updated information or corrections to the original publication.

Summary:

  • The original paper has been amended.
  • An updated version is available.

Impact:

  • Readers should consult the amended version for the most current information.