[Comparative studies on Trichomonas vaginalis in the scanning electron microscope]

Different strains of Trichomonas vaginalis isolated from the vagina of women with trichomoniasis were used in these investigations. The cultures were carried out on modified CPLM and Roiron media. Individuals at the highest phase of logarithmic growth underwent the observation. Preparations to be evaluated in scanning electron microscope ( JEOL - JSM -S1) were made from a condensed culture containing 500,000 individuals per ml. Each time, microscopic pictures obtained after washing, demucination and various fixation procedures and after lyophylisation were compared to each other. The pictures obtained from smears taken from the vagina of women with trichomoniasis were compared, too. Special attention was paid to the behaviour of the surface and motion organelles of the parasite.