Microheterogeneity and structures of neutral glycans present in quail ovomucoid

Anionic chromatography of oligosaccharide-alditols obtained by alkaline hydrolysis of quail ovomucoid glycopeptides resulted in the isolation of a major neutral fraction (83%) which was separated into 14 fractions by high-performance liquid chromatography on an alkylamine silica column. The structures of nine oligosaccharide-alditols were determined. By the identification of mannobiosido-, and mannotriosido-di-N-acetylchitobiose mixed with N-acetyl-lactosaminic and oligomannosidic-types oligosaccharides, we demonstrate the high microheterogeneity of the glycans presents in the neutral fraction of this glycoprotein and suggest that ovomucoid glycosylation uses several pathways.