Probing the interaction of iron complex containing N₃S₂ macrocyclic ligand with bovine serum albumin using...

The interaction of bovine serum albumin (BSA) with seven-coordination iron (II) complex containing sulfur-based macrocyclic ligand was investigated by means of UV/vis absorption spectroscopy and fluorescence quenching technique. The accurate fluorescence spectra are obtained by using Inner filter effect (IFE) correction. The apparent association constant, k_app, the number of binding sites, n, and the apparent binding constant K_SV were found to be 0.95 × 10³ M^-1, 0.96, and 6.13 × 10⁴ M^-1, respectively. It found that BSA molecules are adsorbed on the surface of iron (II) complex by electrostatic interaction. The quenching mechanism is discussed involving energy transfer from BSA to iron (II) complex.