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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

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In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Published on: April 25, 2019

Transcriptomically Guided Mesendoderm Induction of Human Pluripotent Stem Cells Using a Systematically Defined

Richard L Carpenedo¹, Sarah Y Kwon², R Matthew Tanner³

¹The Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada; Ottawa Institute of Systems Biology, Ottawa, ON, Canada.

Stem Cell Reports

|December 10, 2019

Summary

This study presents a simple method using single-cell seeding and BMP4/Activin A treatment for efficient human pluripotent stem cell differentiation into mesendoderm. This protocol is adaptable for mesoderm and endoderm lineage induction.

Keywords:

defined differentiation human embryonic stem cells human induced pluripotent stem cells human pluripotent stem cells mesendoderm

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Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

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Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

Area of Science:

Stem cell biology
Developmental biology
Regenerative medicine

Background:

Human pluripotent stem cells (hPSCs) are crucial for tissue engineering, developmental studies, and disease modeling.
Efficient protocols for rapid hPSC lineage induction are highly sought after in stem cell research.

Purpose of the Study:

To develop a simple, robust, and broadly amenable protocol for differentiating hPSCs into mesendoderm.
To investigate the utility of BMP4 and Activin A (BA) treatment with single-cell seeding (SCS) for lineage induction.

Main Methods:

Differentiation of hPSCs using single-cell seeding (SCS) under defined conditions.
Treatment with Bone Morphogenetic Protein 4 (BMP4) and Activin A (BA).
Fine-tuning of BA treatment conditions for specific lineage commitment.

Main Results:

SCS with BA treatment efficiently induced mesendoderm differentiation.
BA treatment integrated into existing protocols for chondrogenic and endothelial progenitor cell differentiation.
Adjusted BA conditions facilitated definitive endoderm commitment.
Prolonged differentiation showed increased mesoderm/endoderm and decreased ectoderm formation.

Conclusions:

SCS combined with BA treatment is a powerful method for mesendoderm induction from hPSCs.
The protocol is adaptable for mesoderm and endoderm differentiation, offering flexibility in stem cell applications.