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Multipotent stem cell (CFU-S) numbers and circadian variations in aging mice
1Gade Institute, Department of Pathology, Bergen, Norway.
European Journal of Haematology
|September 1, 1988
Summary
Hematopoietic stem cell numbers (CFU-S) decline with age in female mice. Circadian rhythms persist but show reduced amplitude, and seasonal variations affect young mice.
Area of Science:
- Hematology
- Gerontology
- Chronobiology
Background:
- Multipotent stem cell (CFU-S) numbers are crucial for hematopoiesis.
- Aging is associated with physiological changes that may impact stem cell populations.
- Circadian and seasonal variations can influence biological measurements.
Purpose of the Study:
- To investigate the impact of aging on hematopoietic stem cell (CFU-S) numbers in female C3H mice.
- To analyze the circadian variations of CFU-S in young versus aging mice.
- To assess potential seasonal influences on CFU-S numbers and their circadian patterns.
Main Methods:
- Spleen colony assay was used to quantify day-8 CFU-S.
- Measurements were taken every 3 hours over a 24-hour period.
- Studies were conducted on young (3-month-old) and aging (16, 21, 26-month-old) female C3H mice at three different times of the year.
Main Results:
- Significant circadian variations in CFU-S numbers were observed in young mice.
- Aging mice exhibited circadian variations, but with declining peak-trough differences.
- The 24-hour mean CFU-S values consistently decreased with age.
- Young mice showed distinct circadian patterns and mean values depending on the time of year.
Conclusions:
- Day-8 CFU-S numbers significantly decline in aging female mice.
- Aging affects the amplitude of circadian variations in CFU-S.
- Seasonal variations influence CFU-S numbers and rhythms, particularly in younger animals.
- Discrepancies in previous studies may stem from overlooking physiological variations like circadian and seasonal rhythms.