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Downregulated Circular RNA hsa_circ_0000291 Suppresses Migration And Proliferation Of Gastric Cancer Via Targeting

Chuanwu Cao1, Shilong Han1, Yifeng Yuan1

  • 1Department of Interventional and Vascular Surgery, Tenth People's Hospital of Tongji University, Shanghai 200072, People's Republic of China.

Cancer Management and Research
|December 10, 2019
PubMed
Abstract

Insights

Circular RNAs like hsa_circ_0000291 are upregulated in gastric cancer. Silencing hsa_circ_0000291 inhibits cancer growth and metastasis by regulating the miR-183/ITGB1 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in various cancers.
  • hsa_circ_0000291 expression is elevated in gastric cancer (GC) cell lines.
  • The specific function of hsa_circ_0000291 in GC remains largely uncharacterized.

Purpose of the Study:

  • To investigate the functional impact of hsa_circ_0000291 on gastric cancer cell proliferation and invasion.
  • To elucidate the molecular mechanism underlying hsa_circ_0000291's role in GC progression.

Main Methods:

  • Gastric cancer cell lines (MGC803, MKN-28) were utilized to study hsa_circ_0000291.
  • Gene and protein expression analyzed via qRT-PCR and Western blots.
  • MiR-183 sponging activity and ITGB1 regulation assessed using luciferase reporter assays and rescue experiments.
  • In vivo tumor growth and metastasis evaluated in nude mouse xenograft models.

Main Results:

  • hsa_circ_0000291 expression was significantly upregulated in GC cell lines.
  • Silencing hsa_circ_0000291 suppressed both in vitro and in vivo gastric cancer cell proliferation and metastasis.
  • Downregulation of hsa_circ_0000291 led to reduced integrin beta 1 (ITGB1) expression through miR-183 sponging.
  • Rescue experiments confirmed the interaction between hsa_circ_0000291, miR-183, and ITGB1.

Conclusions:

  • Knockdown of hsa_circ_0000291 effectively inhibited gastric cancer cell growth and metastasis.
  • The observed effects are mediated by the regulation of the miR-183/ITGB1 axis.
  • hsa_circ_0000291 presents a potential therapeutic target and diagnostic biomarker for gastric cancer.
Keywords:
gastric cancerhsa_circ_0000291integrin beta 1miR-183proliferation and migration

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