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Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.6K

Circular RNA profile in coronary artery disease

Ren-You Pan1, Chen-Hui Zhao2, Jin-Xia Yuan2

  • 1Department of Cardiovascular Medicine, Yancheng TCM Hospital Affiliated to Nanjing University of Chinese Medicine Yancheng 224000, Jiangsu Province, China.

American Journal of Translational Research
|December 10, 2019
PubMed

Insights

Circular RNAs (circRNAs) are key in coronary artery disease (CAD). This study identified 66 circRNAs in human coronary arteries, suggesting their potential as diagnostic and therapeutic targets for CAD.

Area of Science:

  • Cardiovascular Biology
  • Molecular Genetics
  • Biomarker Discovery

Background:

  • Circular RNAs (circRNAs) are stable, covalently closed molecules with regulatory roles.
  • Their potential as biomarkers and therapeutic targets in coronary artery disease (CAD) is recognized.

Purpose of the Study:

  • To identify and characterize circRNAs in human coronary arteries.
  • To investigate the association between circRNA expression and atherosclerosis.

Main Methods:

  • Autopsy-derived human coronary arteries were analyzed.
  • Histological classification of atherosclerotic lesions using hematoxylin and eosin staining.
  • circRNA expression profiling via RNA sequencing.

Main Results:

  • RNA sequencing identified 1259 annotated and 381 novel circRNAs.
  • Intersection analysis revealed 54 upregulated and 12 downregulated circRNAs in relation to atherosclerosis.
  • Distinct circRNA profiles were observed between segments with and without severe atherosclerosis via hierarchical clustering.

Conclusions:

  • A set of 66 circRNAs are implicated in the pathology of coronary artery atherosclerosis.
  • These identified circRNAs may serve as novel diagnostic or therapeutic targets for CAD.

Keywords:
Coronary arteryRNA sequencingatherosclerosiscircular RNAcoronary artery disease

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