Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.5K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Species interactions are key to spatiotemporal gene expression and multilayer formation in <i>Stenotrophomonas maltophilia</i> K279a dual species biofilms.

Biofilm·2026
Same author

Engineering and Application of a Thermostable MHETase for PET Depolymerization.

ACS sustainable chemistry & engineering·2026
Same author

Molecular characterisation of extended-Spectrum-Beta-lactamase-producing <i>Klebsiella pneumoniae</i> among children and chicken in the rural Korogwe District, Tanzania.

One health (Amsterdam, Netherlands)·2026
Same author

Fluoro4Graphene: A fluorogenic high-throughput screening platform for property engineering of graphene binding peptides.

Talanta·2026
Same author

Defined Nylon Oligomers Enable Mechanistic Insight Into Enzymatic Polyamide Depolymerization.

ChemSusChem·2026
Same author

Genuine Directed Evolution In Test Tube (GENie).

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jan 2, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.4K

Advances in ultrahigh-throughput screening for directed enzyme evolution.

Ulrich Markel1, Khalil D Essani, Volkan Besirlioglu

  • 1Institute of Biotechnology, RWTH Aachen University, Worringer Weg 3, 52074 Aachen, Germany. u.schwaneberg@biotec.rwth-aachen.de.

Chemical Society Reviews
|December 10, 2019
PubMed
Summary

Ultrahigh-throughput screening (uHTS) accelerates enzyme evolution by rapidly assessing millions of variants. This technology is key for discovering novel biocatalysts and optimizing enzymes for industrial applications.

More Related Videos

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.4K
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

9.2K

Related Experiment Videos

Last Updated: Jan 2, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.4K
A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.4K
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

9.2K

Area of Science:

  • Biocatalysis and enzyme engineering
  • Protein engineering and directed evolution
  • Synthetic biology and functional metagenomics

Background:

  • Enzymes are crucial catalysts with vast synthetic potential, often requiring re-engineering for specific applications.
  • Directed evolution and (semi-)rational design are key protein engineering strategies, with directed evolution excelling without structural knowledge.
  • Current enzyme variant libraries exceed 10^12, holding potential for undiscovered catalytic activities and selectivities.

Purpose of the Study:

  • To review recent advancements in ultrahigh-throughput screening (uHTS) for directed enzyme evolution.
  • To highlight the role of compartmentalization in maintaining genotype-phenotype linkage during screening.
  • To explore the application of uHTS in functional metagenomics for identifying novel natural biocatalysts.

Main Methods:

  • Focuses on ultrahigh-throughput screening (uHTS) technologies for enzyme evolution.
  • Discusses compartmentalization strategies using cells and biomimetic systems.
  • Explores the integration of uHTS with functional metagenomics.

Main Results:

  • uHTS significantly accelerates the screening of enzyme variant libraries, overcoming limitations of conventional methods.
  • Compartmentalization techniques (cellular and biomimetic) are vital for preserving genotype-phenotype links.
  • uHTS enables efficient exploration of vast sequence spaces for enzyme optimization and discovery.

Conclusions:

  • Ultrahigh-throughput screening is essential for rapid biocatalyst design and expanding knowledge of enzyme sequence-function relationships.
  • Compartmentalization is a critical enabling technology for effective uHTS in enzyme evolution.
  • uHTS methodologies pave the way for novel functional metagenomics approaches to discover natural enzymes for new chemical transformations.