Related Experiment Video
Updated: Jan 2, 2026
10:59
Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer
Published on: November 2, 2012
23.2K
Metal-Encoded Polystyrene Microbeads as a Mass Cytometry Calibration/Normalization Standard Covering Channels from
Jieyi Liu1, Jonathan Jarzabek1, Daniel Majonis2
1Department of Chemical Engineering and Applied Chemistry , University of Toronto , 200 College Street , Toronto , Ontario Canada M5S 3E5.
Analytical Chemistry
|December 10, 2019
Summary
Researchers developed new microbeads for mass cytometry (MC) calibration. These beads improve data accuracy by enabling better correction of instrumental signal drift in multiparameter cytometric assays.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Mass cytometry (MC) is a powerful technique for multiparameter single-cell analysis using metal isotopes.
- Accurate calibration is crucial for MC data, relying on internal standards like metal-encoded microbeads.
- Current commercial beads lack sufficient elemental coverage for advanced MC detection channels.
Purpose of the Study:
- To develop novel microbeads for enhanced mass cytometry calibration and normalization.
- To address the limitations of existing beads in covering the full spectrum of MC detection channels.
Main Methods:
- Synthesized polystyrene microbeads using multistage dispersion polymerization.
- Encoded microbeads with seven elements: yttrium, indium, bismuth, cerium, europium, holmium, and lutetium.
- Optimized synthesis for uniform size, strong signal intensity, and minimal metal ion leaching.
Main Results:
- Successfully prepared microbeads with eight encoded isotopes (including yttrium, indium, and bismuth).
- Achieved uniform bead size and comparable signal intensities for all encoded isotopes.
- Demonstrated improved precision in normalized MC signals using the novel seven-element beads.
Conclusions:
- The developed seven-element-encoded microbeads provide superior calibration for mass cytometry.
- These novel beads enhance the accuracy and reliability of multiparameter cytometric assays.
- This advancement supports broader application of mass cytometry across various research fields.
