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Published on: March 7, 2018
Highly sensitive voltammetric immunosensor for the detection of prostate specific antigen based on silver nanoprobe
Chidkamon Thunkhamrak1, Prakit Chuntib2, Kontad Ounnunkad3
1Department of Chemistry and Research Laboratory for Analytical Instrument and Electrochemistry Innovation, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; The Graduate School, Chiang Mai University Chiang Mai, 50200, Thailand.
Abstract:
In this work, we developed an effective voltammetric immunosensing platform for the sensitive detection of prostate specific antigen (PSA) utilizing a graphene oxide (GO) modified screen-printed carbon electrode (SPCE) hybridized with the ex-situ prepared silver nanoparticles (AgNPs) as a probe and signal transducer. The sensing platform comprises a direct-type immunoassay involving the selective interaction of PSA with anti-PSA. The surface morphology and analytical performance of the modified SPCE were characterized through relevant instrumentations. The changes in the voltammetric reduction current of AgNPs at 0.11 V in the sensor electrode was correlated to the PSA concentration. Under optimum conditions, the fabricated immunosensor exhibited a sensitive response to PSA with a limit of detection (LOD) of 0.27 ng mL-1 and a dynamic calibration range of 0.75-100.0 ng mL-1. We demonstrated that the participation of AgNPs along with GO modification contribute to the desired signal amplification and sensitive detection of PSA. It is anticipated that the proposed immunosensor can serve as a biomarker and potentially be utilized for the real sample quantification of PSA.
