Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Population pharmacokinetics and clinical evaluation of intravenous acetaminophen and its metabolites in Andalusian horses.

Veterinary journal (London, England : 1997)·2025
Same author

Phenolic enrichment of foods curated in olive oil: Kinetics and chemical evaluation.

Food chemistry: X·2024
Same author

The dynamic changes in olive fruit phenolic metabolism and its contribution to the activation of quiescent Colletotrichum infection.

Food chemistry·2024
Same author

Lipidomics signature in post-COVID patient sera and its influence on the prolonged inflammatory response.

Journal of infection and public health·2024
Same author

Comprehensive profiling of ceramides in human serum by liquid chromatography coupled to tandem mass spectrometry combining data independent/dependent acquisition modes.

Analytica chimica acta·2024
Same author

Qualitative and quantitative determination of phenols and their metabolites in urine by in-syringe solid-phase extraction and LC-MS/MS analysis for evaluation of virgin olive oil metabolism.

Talanta·2023
Same journal

Detection and cross-organ characterization of physiological response to microplastic stress in Panax ginseng based on hyperspectral imaging assisted with machine learning.

Talanta·2026
Same journal

Electron-driven proton transfer behaviors in oxidative DNA lesion 8-oxoguanine: A computational study.

Talanta·2026
Same journal

TiO<sub>2</sub> nanoengineering for high-performance photoelectrochemical biosensors: From fundamental strategies to frontier applications.

Talanta·2026
Same journal

CRISPR-based diagnostics for ESKAPE drug-resistant bacteria: From proof-of-concept to point-of-care.

Talanta·2026
Same journal

Integrated 3D printed wearable microfluidics with removable electrochemical sensor for morphine monitoring in sweat.

Talanta·2026
Same journal

Eu-doped Tourmaline@MOF-ZnO core-shell nanocomposites: Enabling UV-activated room-temperature and ppb-level n-butanol sensing.

Talanta·2026
See all related articles

Related Experiment Video

Updated: Jan 2, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

34.7K

Dry sweat as sample for metabolomics analysis.

M M Delgado-Povedano1, L S Castillo-Peinado1, M Calderón-Santiago1

  • 1Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, Córdoba, Spain; Nanochemistry Research Institute, University of Córdoba, Córdoba, Spain; Maimónides Institute of Biomedical Research (IMIBIC), Reina Sofía University Hospital, Córdoba, Spain; CIBER Fragilidad y Envejecimiento Saludable (CIBERfes), Instituto de Salud Carlos III, Spain.

Talanta
|December 11, 2019
PubMed
Summary
This summary is machine-generated.

Dry sweat collection using filter paper and ethanol-phosphate buffer offers a standardized, non-invasive method for clinical metabolomics. This approach enhances metabolite coverage, identifying unique compounds for disease biomarker discovery.

Keywords:
Dry sweat metabolomeGas chromatographyLiquid chromatographyMass spectrometryMetabolomics analysisSampling protocol

More Related Videos

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

1.0K
One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
09:28

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

13.5K

Related Experiment Videos

Last Updated: Jan 2, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

34.7K
Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

1.0K
One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
09:28

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

13.5K

Area of Science:

  • Clinical metabolomics
  • Biofluid analysis
  • Biomarker discovery

Background:

  • Sweat is a valuable, non-invasive biofluid for clinical metabolomics.
  • Current sweat collection methods lack standardization, often relying on fresh sweat.
  • Dry sweat sampling presents a promising alternative for consistent sample collection.

Purpose of the Study:

  • To compare metabolomics coverage from dry sweat collected on different solid supports and with various solvents.
  • To evaluate dry sweat as a standardized alternative to fresh sweat for metabolite analysis.
  • To identify optimal conditions for dry sweat collection and analysis.

Main Methods:

  • Dry sweat collection using gauzes and filter papers impregnated with different solvents.
  • Analysis of dissolved dry sweat using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
  • Comparison of dry and fresh sweat metabolite profiles from the same individuals.

Main Results:

  • Filter paper with 1:1 ethanol-phosphate buffer yielded the highest metabolomics coverage (175 tentative compounds).
  • Dry sweat uniquely identified metabolites like carnitines, sphingolipids, and N-acyl-amino acids.
  • Dry sweat is superior for low-polarity metabolites; fresh sweat is better for polar compounds.

Conclusions:

  • Dry sweat collection, particularly on filter paper, offers a standardized and effective method for metabolomic analysis.
  • Dry sweat analysis expands metabolite identification, revealing compounds not found in fresh sweat.
  • This method holds potential for developing standardized diagnostic tools for various disorders.