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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Related Experiment Video

Updated: Jan 2, 2026

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus
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Bacterium-Mediated RNA Interference: Potential Application in Plant Protection.

Simon Goodfellow1,2, Daai Zhang1,2, Ming-Bo Wang2

  • 1School of Chemistry and Molecular Bioscience, University of Wollongong, NSW 2522, Australia.

Plants (Basel, Switzerland)
|December 11, 2019
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Summary

Bacterium-mediated RNA interference (bmRNAi) offers a novel, eco-friendly approach for agricultural pest control. This review explores bmRNAi

Keywords:
RNA interferencebacterial-mediated RNAidisease controlplant protection

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Last Updated: Jan 2, 2026

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus
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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Biotechnology

Background:

  • RNA interference (RNAi) presents a sustainable alternative to conventional pesticides for managing agricultural pests and pathogens.
  • Current limitations in RNAi technology stem from the lack of effective external delivery systems for agricultural applications.

Purpose of the Study:

  • To review the current status of bacterium-mediated RNA interference (bmRNAi) for agricultural applications.
  • To identify technical challenges and potential advancements in bmRNAi technology.
  • To discuss the prospective uses of bmRNAi in crop protection strategies.

Main Methods:

  • Review of existing scientific literature on bacterium-mediated RNA interference.
  • Analysis of technical limitations and proposed improvements for bmRNAi systems.
  • Exploration of potential agricultural applications based on current research.

Main Results:

  • Bacterium-mediated RNA interference (bmRNAi) shows significant promise as an externally applied RNAi delivery system for agriculture.
  • Several technical hurdles need to be addressed for efficient and widespread adoption of bmRNAi.

Conclusions:

  • bmRNAi represents a viable and environmentally sound strategy for developing new crop protection methods.
  • Further research and development are crucial to overcome current limitations and fully realize the potential of bmRNAi in agriculture.