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Coronarin D Induces Apoptotic Cell Death and Cell Cycle Arrest in Human Glioblastoma Cell Line

Yollanda E M Franco1,2, Marcia Y Okubo3,4, Adriana D Torre3

  • 1Research Laboratory in Molecular Pharmacology of Bioactive Compounds. São Francisco University, Bragança Paulista 12916-900, SP, Brazil.

Molecules (Basel, Switzerland)
|December 11, 2019
PubMed

Insights

Coronarin D effectively suppressed glioblastoma cell growth by inducing DNA damage and apoptosis. This diterpene shows promise as a novel anticancer agent for brain tumors.

Area of Science:

  • Natural Products Chemistry
  • Molecular Biology
  • Oncology

Background:

  • Glioblastoma (GBM) is an aggressive brain tumor with poor prognosis despite standard treatments.
  • Novel therapeutic agents with improved efficacy and reduced side effects are urgently needed for GBM treatment.

Purpose of the Study:

  • To investigate the anticancer properties of Coronarin D in a human glioblastoma cell line (U-251).
  • To elucidate the molecular mechanisms underlying Coronarin D's anti-glioblastoma effects.

Main Methods:

  • Cell viability assays were performed on U-251 glioblastoma cells treated with Coronarin D.
  • Cell cycle progression, DNA damage markers (p21, H2AX phosphorylation), reactive oxygen species (ROS) production, and mitochondrial membrane potential were analyzed.
  • Apoptosis-related proteins (caspases) and signaling pathways (ERK phosphorylation) were assessed.

Main Results:

  • Coronarin D significantly inhibited U-251 cell viability.
  • The compound induced G1 cell cycle arrest, DNA damage, and apoptosis.
  • Coronarin D increased ROS production, depolarized mitochondrial membrane potential, and activated caspases and ERK signaling.

Conclusions:

  • Coronarin D exhibits potent antiproliferative and pro-apoptotic effects against glioblastoma U-251 cells.
  • The mechanisms involve cell cycle arrest, DNA damage induction, ROS generation, and caspase activation.
  • Coronarin D represents a potential therapeutic candidate for brain cancer treatment, warranting further investigation.
Keywords:
apoptosiscell cycle arrestcoronarin Dglioblastomanatural products

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