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d-Amphetamine and ethanol: a drug-drug interaction study
1Department of Pathology, Texas Tech University School of Medicine, Lubbock 79409.
Brain Research Bulletin
|November 1, 1978
Summary
D-amphetamine noncompetitively inhibits liver aldehyde dehydrogenase (L-ALDH) in vitro and affects liver alcohol dehydrogenase (L-ADH) in vivo, impacting ethanol metabolism differently across species.
Area of Science:
- Pharmacology
- Biochemistry
- Toxicology
Background:
- Ethanol metabolism is primarily regulated by liver alcohol dehydrogenase (L-ADH) and aldehyde dehydrogenase (L-ALDH).
- D-amphetamine is a stimulant drug with known effects on various physiological processes.
Purpose of the Study:
- To investigate the in vivo and in vitro effects of d-amphetamine on L-ADH and L-ALDH enzyme activities.
- To determine if d-amphetamine influences ethanol metabolism in rats and mice.
Main Methods:
- Enzyme activity assays using rat and mouse liver preparations.
- In vivo and in vitro administration of d-amphetamine.
- Measurement of ethanol levels in blood and brain.
Main Results:
- D-amphetamine noncompetitively inhibited L-ALDH in vitro in both species.
- Mitochondrial L-ALDH activity was unaffected by d-amphetamine.
- In vivo, d-amphetamine noncompetitively inhibited mouse L-ADH and reduced ethanol levels in blood and brain.
- Rat L-ADH activity showed a non-significant decline, with less pronounced effects on ethanol metabolism.
Conclusions:
- D-amphetamine exhibits species-specific effects on liver alcohol and aldehyde dehydrogenase activities.
- D-amphetamine can interfere with ethanol metabolism, particularly in mice.
- These findings highlight potential drug-drug interactions involving d-amphetamine and ethanol.