Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Handwashing I: Introduction and Types of Equipment01:18

Handwashing I: Introduction and Types of Equipment

4.8K
Handwashing is hand hygiene with plain or antimicrobial soap and water to physically remove dirt, organic material, and microorganisms. However, it may not kill all microorganisms. The handwashing procedure requires a hand wash basin, liquid soap, paper towels, a domestic waste bin, and disposable nail cleaner as optional equipment.
Hand wash basins in clinical areas should have faucets that can be turned on and off without using the hands; that is, they should be non-touch or lever-operated....
4.8K
Standard Precaution01:26

Standard Precaution

2.7K
Standard precautions are the minimum infection control safeguards used while caring for all patients, irrespective of their disease condition. They help prevent the spread of common infectious microorganisms to healthcare workers, patients, and visitors in all healthcare settings.
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...
2.7K
Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

9.5K
Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
9.5K
Suctioning the Oropharyngeal Airway01:25

Suctioning the Oropharyngeal Airway

700
In preparing for oropharyngeal airway suctioning, a nurse must gather all necessary equipment, including a suction unit with tubing, a prepackaged suction kit, sterile gloves, water or saline for irrigation, a water-soluble lubricant, and additional personal protective equipment (such as a gown, mask, and goggles) to control infections.
After assembling the equipment, the nurse should practice hand hygiene and don appropriate PPE according to infection control guidelines to avoid the...
700
Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

1.0K
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
1.0K
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

861
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
861

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mass Administration of Azithromycin to Infants in Mali to Reduce Mortality.

The New England journal of medicine·2025
Same author

Can ward rounds be used by infection prevention control teams to deliver education and enhance knowledge to clinical staff.

Journal of infection prevention·2025
Same author

Promotion mechanism of nanomaterials and modified/combined nanomaterials on photodegradation of organic pollutants: a review based on meta-analysis.

Journal of environmental management·2025
Same author

Effects of nanoplastics and compound pollutants containing nanoplastics on plants, microorganisms and rhizosphere systems: A review.

Ecotoxicology and environmental safety·2025
Same author

Interventions used to reduce infectious aerosol concentrations in hospitals-a review.

EClinicalMedicine·2025
Same author

Antibiotic Residues in UK Foods: Exploring the Exposure Pathways and Associated Health Risks.

Toxics·2024

Related Experiment Video

Updated: Jan 2, 2026

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
09:30

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

4.3K

Surface sampling within a pediatric ward-how multiple factors affect cleaning efficacy.

Stacey Rawlinson1, Elaine Cloutman-Green2, Faiza Asadi3

  • 1Department of Civil, Environmental and Geomatic Engineering, University College London, London, UK.

American Journal of Infection Control
|December 11, 2019
PubMed
Summary

This study assessed microbial contamination on surfaces in a pediatric ward before and after cleaning. Cleaning significantly reduced colony-forming units (CFUs) by 68%, highlighting the need for improved cleaning protocols.

Keywords:
CleaningEnvironmental monitoringHealth care–associated infectionsHospitalInfection prevention and controlSurface sampling

More Related Videos

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
09:02

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens

Published on: June 7, 2017

19.6K
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

17.7K

Related Experiment Videos

Last Updated: Jan 2, 2026

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
09:30

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

4.3K
A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
09:02

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens

Published on: June 7, 2017

19.6K
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

17.7K

Area of Science:

  • Clinical Microbiology
  • Infection Prevention and Control
  • Environmental Hygiene

Background:

  • Assessing microbial load on surfaces in healthcare settings is crucial for infection control.
  • Pediatric oncology wards present unique challenges due to vulnerable patient populations.

Purpose of the Study:

  • To quantify microbial contamination on various surfaces in a pediatric ward.
  • To evaluate the effectiveness of standard cleaning procedures in reducing microbial load.
  • To identify factors influencing cleaning efficacy.

Main Methods:

  • Conducted a 2-week microbiological environmental monitoring study in a pediatric hematology-oncology ward.
  • Collected 1,160 surface samples using tryptone soya agar contact plates from 55 sites before and after cleaning.
  • Sampled diverse surfaces considering material, height, function, patient proximity, and touch frequency.

Main Results:

  • A statistically significant reduction in colony-forming units (CFUs) was observed post-cleaning (P < .0001).
  • Cleaning achieved an average CFU reduction of 68% across the ward.
  • Corridors were the most contaminated areas, and cleaning efficiency varied by ward area and surface characteristics.

Conclusions:

  • This study is the first to directly assess cleaning effectiveness in a pediatric ward using pre- and post-cleaning sampling.
  • Findings indicate a need for enhanced cleaning interventions and highlight the importance of considering multiple factors in cleaning protocol design.
  • The study provides insights into optimizing cleaning strategies to improve environmental hygiene in pediatric healthcare settings.