Elevating performance of electrochemical immunosensor via photo-induced microscale hyperthermia in situ

Catalytic reactions contribute a lot to electrochemical sensing by amplifying electrochemical signals to elevating sensitivity, allowing ultrasensitive sensing of bioindicators. However, the unsatisfactory catalytical performance of catalysts results in low efficiency, limiting its practice in rapid immunosensing. Herein, we demonstrated the potential of photo-induced microscale hyperthermia in accelerating catalysis to enhance sensitivity in the short time. Under near-infrared (NIR) laser irradiation, the period of Fenton-like reaction was significantly reduced, further allowing rapid change of electrical signal on electrode in situ. By constructing a novel immunosensor, efficacious sensing of Squamous Cell Carcinoma Antigen (SCCA) was achieved with improved sensitivity for two times, high timeliness within several minutes and advanced performance of electrochemical immunosensor (linear detection range: 0.1 pg mL^-1-1 μg mL^-1; limit of detection: 120.2 fg mL^-1). To the best of our knowledge, this research is the first work that typifies the photothermal-enhanced catalysis in the electrochemical immunoassays, which illuminates a great direction of developing advanced electrochemical sensing protocol with both favorable capacities and accelerated process.