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Updated: Jan 2, 2026

Vertical Immobilization Method for Time-Lapse Microscopy Analysis in Filamentous Cyanobacteria
Published on: September 25, 2023
Intracellular offspring released from SFB filaments are flagellated
Iris Nkamba1,2, Céline Mulet3, Gyanendra P Dubey3
1Institut Imagine, Laboratory of Intestinal Immunity, Inserm UMR1163, Paris, France.
None:
The gut commensal segmented filamentous bacterium (SFB) attaches to the ileal epithelium and potently stimulates the host immune system. Using transmission electron microscopy (TEM), we show that mouse and rat SFB are flagellated above the concave tip at the unicellular intracellular offspring (IO) stage and that flagellation occurs prior to full IO differentiation and release of IOs from SFB filaments. This finding adds a missing link to the SFB life cycle.
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