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Updated: Jan 2, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Localization microscopy at doubled precision with patterned illumination
Jelmer Cnossen1,2, Taylor Hinsdale1, Rasmus Ø Thorsen1
1Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
Abstract:
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.
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