Related Experiment Video
Updated: Jan 2, 2026
Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice
Published on: March 17, 2014
Sputum Exosomal microRNAs Profiling Reveals Critical Pathways Modulated By Pseudomonas aeruginosa Colonization In
Yan Huang1, Chun-Lan Chen1, Jing-Jing Yuan1
1Department of Respiratory Medicine, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute for Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, People's Republic of China.
Background:
Pseudomonas aeruginosa (PA) colonization confers poor prognosis in bronchiectasis. However, the biomarkers and biological pathways underlying these associations are unclear.
Objective:
To identify the roles of PA colonization in bronchiectasis by exploring for sputum exosomal microRNA profiles.
Methods:
We enrolled 98 patients with clinically stable bronchiectasis and 17 healthy subjects. Sputum was split for bacterial culture and exosomal microRNA sequencing, followed by validation with quantitative polymerase chain reaction. Bronchiectasis patients were stratified into PA and non-PA colonization groups based on sputum culture findings. We applied Gene Ontology and Kyoto Encyclopedia of Genes and Genome pathway enrichment analysis to explore biological pathways corresponding to the differentially expressed microRNAs (DEMs) associated with PA colonization.
Results:
Eighty-two bronchiectasis patients and 9 healthy subjects yielded sufficient sputum that passed quality control. We identified 10 overlap DEMs for the comparison between bronchiectasis patients and healthy subjects, and between PA and non-PA colonization group. Both miR-92b-5p and miR-223-3p could discriminate PA colonization (C-statistic >0.60) and independently correlated with PA colonization in multiple linear regression analysis. The differential expression of miR-92b-5p was validated by quantitative polymerase chain reaction (P<0.05), whereas the differential expression of miR-223 trended towards statistical significance (P=0.06). These DEMs, whose expression levels correlated significantly with sputum inflammatory biomarkers (interleukin-1β and interleukin-8) level, were implicated in the modulation of the nuclear factor-κB, phosphatidylinositol and longevity regulation pathways.
Conclusion:
Sputum exosomal microRNAs are implicated in PA colonization in bronchiectasis, highlighting candidate targets for therapeutic interventions to mitigate the adverse impacts conferred by PA colonization.
Insights
Pseudomonas aeruginosa colonization worsens bronchiectasis outcomes. Sputum exosomal microRNAs, including miR-92b-5p and miR-223-3p, are linked to this colonization and associated inflammation.
Area of Science:
- Pulmonary Medicine
- Molecular Biology
- Microbiology
Background:
- Pseudomonas aeruginosa (PA) colonization is linked to poor prognosis in bronchiectasis.
- The specific biomarkers and biological pathways involved remain unclear.
Purpose of the Study:
- To investigate the role of PA colonization in bronchiectasis by analyzing sputum exosomal microRNA profiles.
- To identify potential biomarkers associated with PA colonization in bronchiectasis patients.
Main Methods:
- Analysis of sputum exosomal microRNA sequencing in 98 bronchiectasis patients and 17 healthy controls.
- Stratification of bronchiectasis patients into PA and non-PA colonization groups.
- Gene Ontology and KEGG pathway enrichment analysis to explore biological pathways of differentially expressed microRNAs (DEMs).
Main Results:
- Ten overlap DEMs were identified between bronchiectasis patients and healthy subjects, and between PA and non-PA groups.
- miR-92b-5p and miR-223-3p could discriminate PA colonization and correlated with it.
- DEMs were implicated in nuclear factor-κB, phosphatidylinositol, and longevity regulation pathways, correlating with inflammatory biomarkers.
Conclusions:
- Sputum exosomal microRNAs play a role in PA colonization in bronchiectasis.
- These microRNAs represent potential therapeutic targets to manage the adverse effects of PA colonization.
More Related Videos
03:53Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes
Published on: April 19, 2024
10:26P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
Published on: June 15, 2020
Related Concept Videos
01:26Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
01:28Gene Regulation in Microbial Communities: Quorum Sensing
01:29Pneumonia II: Pathophysiology