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Updated: Jan 2, 2026
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Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
Published on: January 7, 2013
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TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ
Yong Yang1, Jinhai Fan2, Shenglu Han1
1Department of Urology, The Ninth Hospital of Xi'an, Xi'an, Shaanxi, People's Republic of China.
Oncotargets and Therapy
|December 11, 2019
Summary
Tumor suppressor TNIP1 (TNF-α-induced protein 3-interacting protein 1) is downregulated in clear cell renal cell carcinoma (ccRCC). Its loss promotes ccRCC cell proliferation and inhibits apoptosis by affecting C/EBPβ expression.
Area of Science:
- Oncology
- Molecular Biology
Background:
- Renal cell carcinoma (RCC) is a significant cancer with limited therapeutic options.
- Clear cell renal cell carcinoma (ccRCC) is the most common subtype with a poor prognosis.
- The role of TNF-α-induced protein 3-interacting protein 1 (TNIP1) in ccRCC remains uncharacterized.
Purpose of the Study:
- To investigate the expression and function of TNIP1 in ccRCC.
- To determine the potential of TNIP1 as a diagnostic marker and therapeutic target in ccRCC.
Main Methods:
- TNIP1 expression analyzed via qRT-PCR, Western blot, and IHC in ccRCC tissues and cells.
- Cell proliferation assessed using CCK-8 assay.
- Cell cycle and apoptosis evaluated by flow cytometry.
Main Results:
- TNIP1 expression is significantly downregulated in ccRCC tissues and cells.
- TNIP1 downregulation enhances ccRCC cell proliferation and cell cycle progression.
- TNIP1 downregulation inhibits apoptosis by upregulating Bcl-2 and downregulating Bax and cleaved-caspase-3 via C/EBPβ.
- TNIP1 acts as a tumor suppressor in ccRCC by targeting C/EBPβ.
Conclusions:
- TNIP1 functions as a tumor suppressor in ccRCC.
- TNIP1 downregulation is linked to increased proliferation and decreased apoptosis.
- TNIP1 represents a potential diagnostic marker and therapeutic target for ccRCC.
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