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JAZF1 Suppresses Papillary Thyroid Carcinoma Cell Proliferation and Facilitates Apoptosis via Regulating TAK1/NF-κB

Liangliang Huang1, Yuhuai Cai1, Yi Luo1

  • 1Medical Center of Breast and Thyroid Disease, Affiliated Hospital of ZunYi Medical University, ZunYi, Guizhou 563003, People's Republic of China.

Oncotargets and Therapy
|December 11, 2019
PubMed
Abstract

Insights

Juxtaposed with another zinc finger gene 1 (JAZF1) is downregulated in papillary thyroid cancer (PTC). Overexpressing JAZF1 inhibits PTC cell proliferation and induces apoptosis by suppressing TAK1/NF-κB signaling, suggesting JAZF1 as a potential PTC marker.

Area of Science:

  • Molecular biology
  • Cancer research
  • Endocrinology

Background:

  • Juxtaposed with another zinc finger gene 1 (JAZF1) plays roles in various cellular processes but its function in carcinoma is unclear.
  • Papillary thyroid cancer (PTC) is the most common type of thyroid cancer, necessitating research into novel molecular markers and therapeutic targets.

Purpose of the Study:

  • To investigate the expression and function of JAZF1 in human papillary thyroid cancer (PTC).
  • To explore the potential of JAZF1 as a diagnostic marker and therapeutic target in PTC.

Main Methods:

  • Immunohistochemistry, Western blotting, and RT-qPCR were used to assess JAZF1 expression in PTC tissues and cell lines.
  • Functional assays including cell proliferation, colony formation, cell cycle, and apoptosis analyses were performed in JAZF1-overexpressing BCPAP cells.
  • The study examined the impact of JAZF1 on the TAK1/NF-κB signaling pathway.

Main Results:

  • JAZF1 expression was significantly downregulated in PTC tissues compared to adjacent normal tissues and nodular goitres.
  • JAZF1 expression levels negatively correlated with Ki67 labelling index and were associated with tumor location and lymph node metastasis.
  • Overexpression of JAZF1 in BCPAP cells inhibited proliferation, induced G0/G1 cell cycle arrest, promoted apoptosis, and suppressed TAK1/NF-κB signaling.

Conclusions:

  • JAZF1 inhibits PTC cell proliferation and induces apoptosis by suppressing the TAK1/NF-κB signaling pathway.
  • JAZF1 may serve as a valuable molecular marker for the diagnosis and potentially the treatment of PTC.
Keywords:
Juxtaposed with another zinc finger gene 1NF-κBTAK1papillary thyroid cancer

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