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Related Experiment Video

Updated: Jan 2, 2026

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Antibody Screening.

Fieke W Hoff¹, Yiling Lu², Steven M Kornblau³

¹Department of Pediatric Oncology/Hematology, Beatrix Children's Hospital, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

Advances in Experimental Medicine and Biology

|December 11, 2019

Summary

This summary is machine-generated.

Antibody validation is critical for reliable research, especially in reverse phase protein arrays (RPPA). Current methods are insufficient, highlighting the need for standardized antibody validation strategies to ensure accuracy and reproducibility in scientific discovery.

Keywords:

Antibody Proteomics Reverse phase protein array (RPPA)Screening Validation

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Last Updated: Jan 2, 2026

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Published on: August 22, 2019

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Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Published on: September 10, 2018

Area of Science:

Biotechnology
Immunology
Proteomics

Background:

Antibodies are essential research tools impacting diagnostics, therapeutics, and disease understanding.
High specificity, selectivity, and reproducibility of antibodies are crucial for applications like reverse phase protein arrays (RPPA).
Numerous studies reveal significant issues with antibody reliability, including lack of specificity, application-dependent functionality, and instability across batches and time.

Purpose of the Study:

To emphasize the critical need for robust antibody validation in scientific research.
To review current antibody validation strategies employed in RPPA.
To propose a novel strategy for reporting, scoring, and integrating antibody validation data from multiple sources.

Main Methods:

Discussion of existing antibody validation challenges and limitations.
Review of current practices for antibody validation in RPPA.
Proposal of a new framework for antibody validation data management.

Main Results:

Identified widespread issues with antibody specificity, reproducibility, and stability.
Highlighted the lack of universally accepted antibody validation guidelines.
Presented a new strategy for comprehensive antibody validation reporting and scoring.

Conclusions:

There is an urgent and unmet need for standardized antibody validation protocols.
Current validation methods for RPPA are insufficient to guarantee antibody performance.
A new, integrated approach to antibody validation is proposed to enhance research reliability and reproducibility.