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Related Experiment Video

Updated: Jan 2, 2026

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
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Utility of Reverse-Phase Protein Array for Refining Precision Oncology.

Mari Masuda1, Tesshi Yamada2

  • 1Division of Cellular Signaling, National Cancer Center Research Institute, Tokyo, Japan. mamasuda@ncc.go.jp.

Advances in Experimental Medicine and Biology
|December 11, 2019
PubMed
Summary

Genomic profiling alone limits precision oncology. Proteomics, particularly Reverse-Phase Protein Array (RPPA), offers a more comprehensive approach to match cancer treatments by analyzing protein expression and signaling pathways.

Keywords:
BiomarkerCompanion diagnosticsDrug resistancePrecision oncologyReverse-phase protein arrayTissue-agnostic biomarker

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Area of Science:

  • Oncology
  • Genomics
  • Proteomics

Background:

  • Genomics-driven precision oncology has shown limited success for many cancer patients.
  • Current genomic profiling is insufficient for optimal targeted therapy selection.
  • Proteomics captures biological processes crucial for cancer pathogenesis, unlike genomics or transcriptomics.

Purpose of the Study:

  • To review the limitations of current genomics-driven precision oncology.
  • To highlight the potential of proteomics-based approaches in cancer treatment.
  • To discuss the utility of Reverse-Phase Protein Array (RPPA) technology for advancing precision oncology.

Main Methods:

  • Review of genomics-driven precision oncology status and challenges.
  • Discussion of proteomics-based approaches, including protein expression and post-translational modifications.
  • Evaluation of Reverse-Phase Protein Array (RPPA) for pharmacodynamic analysis and clinical utility.

Main Results:

  • Genomic profiling alone has significant limitations in patient selection for targeted therapies.
  • Proteomics directly captures key biological processes involved in cancer development.
  • RPPA technology enables precise mapping of signaling status with limited clinical samples.

Conclusions:

  • Proteomic analyses should be incorporated into the next phase of precision oncology.
  • RPPA technology is a cost-effective and rapid platform suitable for clinical settings.
  • RPPA offers advantages over existing molecular profiling technologies for improving precision oncology outcomes.