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Magnesium-dependent activated partial thromboplastin time assay-Simple method for lupus anticoagulant detection
Takayoshi Tokutake1, Masahiro Ieko2, Sumiyoshi Naito3
1Department of Transfusion, Nagano Red Cross Hospital, Nagano, Japan.
International Journal of Laboratory Hematology
|December 11, 2019
Summary
A new Mg-APTT assay offers a simple and highly specific method for detecting lupus anticoagulant (LA), an antibody crucial for diagnosing antiphospholipid syndrome (APS). This test provides accurate results with 100% specificity.
Area of Science:
- Hematology
- Immunology
- Clinical Chemistry
Background:
- Lupus anticoagulant (LA) detection is vital for diagnosing antiphospholipid syndrome (APS).
- Current LA confirmation methods are often complex and time-consuming.
- A need exists for simpler, more specific LA detection assays.
Purpose of the Study:
- To introduce and evaluate a novel, simplified method for lupus anticoagulant detection.
- To assess the diagnostic performance of the Mg-APTT assay for LA identification.
Main Methods:
- Evaluated 66 LA-positive and 75 LA-negative plasma samples, plus 43 healthy controls.
- Measured activated partial thromboplastin time (APTT) using CaCl2 (Ca-APTT) and a Mg-Ca mixture (Mg-APTT).
- Calculated the Mg/Ca Index (Mg-APTT/Ca-APTT ratio) for LA assessment.
Main Results:
- The Mg/Ca Index was significantly lower in the LA-positive group compared to non-LA and control groups (P < .0001).
- Using a cutoff value < 1.00, the Mg-APTT assay demonstrated 80.3% sensitivity for LA detection.
- The assay achieved 100% specificity in distinguishing LA-positive from LA-negative samples.
Conclusions:
- The Mg-APTT assay presents a straightforward and highly specific diagnostic tool for lupus anticoagulant.
- This method simplifies LA detection, potentially improving the diagnostic workflow for APS.
