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Related Experiment Videos

Spiramycin uptake by alveolar macrophages.

R Harf1, G Panteix, J F Desnottes

  • 1Centre Hospitalier Lyon-Sud, Pierre-Benite, France.

The Journal of Antimicrobial Chemotherapy
|July 1, 1988
PubMed
Summary

Spiramycin effectively penetrates human and animal alveolar macrophages, reaching high concentrations within lung tissues. This antibiotic demonstrates significant uptake and sustained presence in the alveolar space, supporting its use in respiratory infections.

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Area of Science:

  • Pharmacology
  • Infectious Diseases
  • Pulmonology

Background:

  • Alveolar macrophages play a crucial role in lung defense.
  • Understanding antibiotic penetration into macrophages is vital for treating respiratory infections.

Purpose of the Study:

  • To investigate the in-vitro and in-vivo uptake of spiramycin by human and animal alveolar macrophages.
  • To determine spiramycin's penetration into the alveolar space and its concentration within macrophages.

Main Methods:

  • In-vitro studies using guinea pig and human alveolar macrophages incubated with spiramycin.
  • In-vivo studies in patients receiving spiramycin infusions, with serum and bronchoalveolar lavage (BAL) sampling.
  • Spiramycin quantification using High-Performance Liquid Chromatography (HPLC) and microbiological assays.

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Main Results:

  • High cellular/extracellular (C/E) concentration ratios for spiramycin were observed in both guinea pig (20.3) and human (21.3-23.8) alveolar macrophages.
  • Spiramycin demonstrated slow release from macrophages after initial uptake.
  • In vivo, BAL supernatant spiramycin levels were approximately 24-fold higher than serum levels, with macrophage levels ranging from 17 to 210 mg/l.

Conclusions:

  • Spiramycin exhibits excellent penetration into alveolar macrophages.
  • The drug accumulates significantly within the alveolar space, supporting its therapeutic potential for lung infections.