Distinct Features of Canine Non-conventional CD4^-CD8α^- Double-Negative TCRαβ⁺ vs. TCRγδ⁺ T Cells

The role of conventional TCRαβ⁺CD4⁺ or TCRαβ⁺CD8α⁺ single-positive (sp) T lymphocytes in adaptive immunity is well-recognized. However, non-conventional T cells expressing TCRαβ or TCRγδ but lacking CD4 and CD8α expression [i.e., CD4^-CD8α^- double-negative (dn) T cells] are thought to play a role at the interface between the innate and adaptive immune system. Dn T cells are frequent in swine, cattle or sheep and predominantly express TCRγδ. In contrast, TCRγδ⁺ T cells are rare in dogs. In this study, we identified a high proportion of canine dn T cells in the TCRαβ⁺ T cell population of PBMC, lymphatic and non-lymphatic organs. In PBMC, the frequency of this T cell subpopulation made up one third of the frequency of TCRαβ⁺CD4⁺ sp, and almost half of the frequency of TCRαβ⁺CD8α⁺ sp T cells (i.e., ~15% of all TCRαβ⁺ T cells). Among TCRαβ⁺CD4^-CD8α^- dn T cells of PBMC and tissues, FoxP3⁺ cells were identified indicating regulatory potential of this T cell subset. 80% of peripheral blood FoxP3⁺TCRαβ⁺CD4^-CD8α^- dn T cells co-expressed CD25, and, interestingly, also the FoxP3-negative TCRαβ⁺CD4^-CD8α^- dn T cells comprised ~34% CD25⁺ cells. Some of the FoxP3-positive TCRαβ⁺CD4^-CD8α^- dn T cells co-expressed GATA-3 suggesting stable function of regulatory T cells. The frequency of GATA-3 expression by FoxP3^-TCRαβ⁺CD4^-CD8α^- dn T cells was even higher as compared with TCRαβ⁺CD4⁺ sp T cells (20.6% vs. 11.9%). Albeit lacking FoxP3 and CD25 expression, TCRγδ⁺CD4^-CD8α^- dn T cells also expressed substantial proportions of GATA-3. In addition, TCRαβ⁺CD4^-CD8α^- dn T cells produced IFN-γ and IL-17A upon stimulation. T-bet and granzyme B were only weakly expressed by both dn T cell subsets. In conclusion, this study identifies two dn T cell subsets in the dog: (i) a large (~7.5% in Peyer's patches, ~15% in lung) population of TCRαβ⁺CD4^-CD8α^- dn T cells with subpopulations thereof showing an activated phenotype, high expression of FoxP3 or GATA-3 as well as production of IFN-γ or IL-17A and (ii) a small TCRγδ⁺CD4^-CD8α^- dn T cell subset also expressing GATA-3 without production of IFN-γ or IL-17A. It will be exciting to unravel the function of each subset during immune homeostasis and diseases of dogs.