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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
MiR-9-5p Inhibits Glioblastoma Cells Proliferation Through Directly Targeting FOXP2 (Forkhead Box P2)
Hongbo Zhang1,2, Yuntao Li3,4, Yinqiu Tan3
1Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Abstract:
Glioblastoma (GBM) is the most malignant tumor in the central nervous system and the treatment is still unsatisfactory because the mechanism of the disease remains unclear. The abnormal expression of miRNAs and its target proteins play a crucial role in the development of glioblastoma. In this study, we demonstrated that high expression of miR-9-5p and low expression of forkhead box P2 (FOXP2) were related with better outcome in patients with GBM, and down regulated FOXP2 expression was able to inhibit glioma cells proliferation by cell cycle arrest. Furthermore, we found that FOXP2 was the target protein of miR-9-5p in luciferase assay. The results of this study suggest a novel regulatory mechanism that miR-9-5p can inhibit glioma cells proliferation by downregulating FOXP2.
Insights
High miR-9-5p and low forkhead box P2 (FOXP2) expression correlate with better glioblastoma outcomes. MiR-9-5p inhibits glioma cell growth by targeting and downregulating FOXP2.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Cancer Research
Background:
- Glioblastoma (GBM) is a highly aggressive brain tumor with poor prognosis.
- The underlying molecular mechanisms of GBM remain incompletely understood.
- MicroRNAs (miRNAs) and their target proteins are implicated in GBM development.
Purpose of the Study:
- To investigate the role of miR-9-5p and forkhead box P2 (FOXP2) in glioblastoma.
- To elucidate the regulatory relationship between miR-9-5p and FOXP2 in glioma cells.
Main Methods:
- Analysis of miR-9-5p and FOXP2 expression in GBM patient samples.
- Assessment of FOXP2's effect on glioma cell proliferation and cell cycle.
- Luciferase reporter assays to confirm direct targeting of FOXP2 by miR-9-5p.
Main Results:
- High miR-9-5p expression and low FOXP2 expression were associated with improved patient survival.
- Downregulation of FOXP2 inhibited glioma cell proliferation via cell cycle arrest.
- FOXP2 was identified as a direct target of miR-9-5p.
Conclusions:
- miR-9-5p acts as a tumor suppressor in glioblastoma by targeting FOXP2.
- This miR-9-5p/FOXP2 axis represents a potential therapeutic target for GBM.
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