Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuronal CCL2 responds to hyperglycaemia and contributes to anxiety disorders in the context of diabetes.

Nature metabolism·2025
Same author

Accurate measurement of oxygen consumption in children undergoing cardiac catheterization.

Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions·2012
Same author

Glutathione S-transferase polymorphisms and bone tumor risk in China.

Asian Pacific journal of cancer prevention : APJCP·2012
Same author

Systemic oxygen transport derived by using continuous measured oxygen consumption after the Norwood procedure-an interim review.

Interactive cardiovascular and thoracic surgery·2012
Same author

Discovery and optimization of 2,4-diaminoquinazoline derivatives as a new class of potent dengue virus inhibitors.

Journal of medicinal chemistry·2012
Same author

β(3)-Adrenoceptor Antagonist SR59230A Attenuates the Imbalance of Systemic and Myocardial Oxygen Transport Induced by Dopamine in Newborn Lambs.

Clinical Medicine Insights. Cardiology·2012

Related Experiment Video

Updated: Jan 2, 2026

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
13:47

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

11.2K

A fluorogenic probe for tracking GSH flux in developing neurons.

Haichao Zong1, Jiayi Peng, Xiao-Rong Li

  • 1State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. yzang@simm.ac.cn.

Chemical Communications (Cambridge, England)
|December 12, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a sensitive new probe to measure glutathione (GSH) changes in developing neurons, crucial for understanding their growth and function.

More Related Videos

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
08:59

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

3.0K
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
10:30

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Published on: January 12, 2014

8.7K

Related Experiment Videos

Last Updated: Jan 2, 2026

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
13:47

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

11.2K
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
08:59

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

3.0K
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
10:30

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Published on: January 12, 2014

8.7K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Glutathione (GSH) plays a critical role in cellular processes, including neuronal development.
  • Understanding GSH dynamics is essential for elucidating its function in developing neurons.
  • Existing methods lack the sensitivity to detect subtle GSH fluctuations in live cells.

Purpose of the Study:

  • To develop an ultrasensitive assay for imaging glutathione (GSH) flux in developing neurons.
  • To investigate the role of GSH in neuronal development by enabling real-time monitoring.
  • To identify key factors influencing probe sensitivity for live-cell imaging.

Main Methods:

  • Systematic exploration of structural factors affecting fluorogenic probe sensitivity.
  • Development and validation of a novel fluorogenic probe for GSH detection.
  • Live-cell imaging of GSH fluctuations in developing neurons.

Main Results:

  • A highly sensitive fluorogenic probe for imaging GSH was successfully developed.
  • The probe enabled visualization of subtle GSH fluctuations in developing neurons.
  • Key structural determinants of probe sensitivity in live cells were identified.

Conclusions:

  • The developed ultrasensitive probe is a valuable tool for studying GSH dynamics in neuronal development.
  • This advancement facilitates a deeper understanding of GSH's role in the nervous system.
  • The findings pave the way for future investigations into neurodevelopmental processes involving GSH.