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MicroRNA profiling in canine multicentric lymphoma
Karlee K L Craig1, Geoffrey A Wood1, Stefan M Keller1
1Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Plos One
|December 12, 2019
Summary
This study identified specific microRNAs (miRNAs) in dogs with lymphoma. Altered miRNA expression in lymph nodes and plasma may serve as biomarkers for diagnosis and predicting treatment outcomes in canine lymphoma.
Area of Science:
- Veterinary Oncology
- Molecular Biology
- Cancer Genomics
Background:
- Lymphoma is a common canine hematopoietic tumor, sharing similarities with human disease.
- MicroRNAs (miRNAs) are key regulators of gene expression, and their dysregulation is implicated in cancer development.
- Biomarker discovery is crucial for improving diagnostics and predicting therapeutic responses in canine lymphoma.
Purpose of the Study:
- To characterize microRNA expression profiles in canine multicentric lymphoma (B and T cell types) in lymph nodes and plasma.
- To compare miRNA expression between lymph nodes and plasma, and assess changes from diagnosis to relapse.
- To investigate the association of specific microRNAs with clinical outcomes in dogs treated with chemotherapy.
Main Methods:
- Utilized a customized PCR array to profile 38 canine microRNAs.
- Quantified microRNA expression using real-time RT-qPCR and the delta-delta Ct method.
- Analyzed differential expression in lymph nodes and plasma, and correlated expression with treatment response and survival.
Main Results:
- Significant alterations in microRNA expression were observed in both lymph nodes and plasma of dogs with lymphoma compared to controls.
- Limited correlation in microRNA expression was found between lymph node and plasma samples.
- Differential microRNA expression was associated with treatment non-remission and survival outcomes, with several miRNAs linked to progression-free and overall survival.
Conclusions:
- Specific microRNAs show altered expression patterns in canine multicentric lymphoma, suggesting their potential as diagnostic and prognostic biomarkers.
- MicroRNA expression in plasma may offer a less invasive approach for monitoring disease status.
- Further development of microRNA-based panels could enhance outcome prediction for veterinary cancer patients.
